Protein Expression

Proteins, the primary executors of life activities, are crucial for understanding biological phenomena and developing novel therapeutics. Acting as efficient "protein factory," Tingeibio is capable of expressing and purifying various target proteins according to your needs, providing high-quality protein samples to accelerate your research progress.

Service Process:

1. Requirement Confirmation: Clients provide the target protein gene sequence, expression system, purification tags, and other relevant information.

2. Vector Construction: The target gene is cloned into an expression vector and transformed into a host expression cell line.

3. Protein Expression: Expression conditions are optimized to induce target protein expression.

4. Protein Purification: Target proteins are purified using methods such as affinity chromatography, ion exchange chromatography, and size exclusion chromatography.

5. Protein Characterization: Protein molecular weight, purity, and activity are verified using SDS-PAGE, Western Blot, mass spectrometry, and other techniques.

6. Product Delivery: Purified and characterized protein products are delivered to the client along with a quality control report.

Service Type:

• Prokaryotic Expression Systems: E. coli expression systems, known for their simplicity and cost-effectiveness, are suitable for expressing soluble proteins.

• Eukaryotic Expression Systems: Mammalian cell, insect cell, and yeast expression systems are ideal for proteins requiring complex modifications.

• Cell-Free Expression Systems: Fast and efficient, suitable for expressing toxic proteins, membrane proteins, and other challenging targets.

• Large-Scale Protein Expression: Offers scalable protein expression from shake flasks to bioreactors, meeting industrial production needs.

Our Advantage:

• Comprehensive Expression Systems: A variety of prokaryotic and eukaryotic expression systems to meet diverse protein expression requirements.

• Extensive Purification Expertise: Multiple protein purification techniques to handle various protein types.

• Stringent Quality Control: Every step, from gene sequence to final product, undergoes rigorous quality control to ensure protein quality.

• Fast and Efficient Delivery: Experienced technical teams and advanced equipment enable rapid completion of expression tasks.

• Comprehensive After-Sales Support: Detailed product quality reports and technical support to address any concerns.

Application:

• Protein Structure and Function Studies: X-ray crystallography, nuclear magnetic resonance (NMR), cryo-electron microscopy (Cryo-EM), etc.

• Antibody Drug Development: Monoclonal antibodies, bispecific antibodies, antibody-drug conjugates, etc.

• Vaccine Development: Recombinant protein vaccines, virus-like particle vaccines, etc.

• Enzyme Engineering: Enzyme directed evolution, enzyme immobilization, etc.

At Tingeibio, we ensure every client 3 points below,

1. PhD level customer support;
2. Excellent and stable quality;
3. Timely delivery.

How to Order:

Please download Protein Expression Order Form, fill it out and then send it to service@tingeibio.com.